The RND permease superfamily: an ancient, ubiquitous and diverse family that includes human disease and development protein. How to say Alcanivorax borkumensis in English? 1998 [1] Type strain. How effective is in-situ burning? Purification and properties of dihydroneopterin aldolase. PubMed Cells were grown in LB medium with 100gml1 ampicillin at 37C. J Mol Microbiol Biotechnol 1:6370, CAS 2019 Feb 18;19(1):41. doi: 10.1186/s12866-019-1406-x. Sabirova JS, Chernikova TN, Timmis KN, Golyshin PN. Dispersants are thought to be important in the creation of oil . Alcanivorax borkumensis is an oil-degrading marine bacterium. Its genome contains genes coding for three cytochrome P450s and two integral membrane alkane hydroxylases (AlkB1 & AlkB2), all assumed to perform hydroxylation of different linear or branched alkanes. Yakimov et al. . & Nichols, B. P. Nucleotide sequence of Escherichia coli pabA and its evolutionary relationship to trp(G)D. J. Mol. Google Scholar, Rozhkova-Novosad EA, Chae JC, Zylstra GJ, Bertrand EM, Alexander-Ozinskas M, Deng D, Moe LA, van Beilen JB, Danahy M, Groves JT, Austin RN (2007) Profiling mechanisms of alkane hydroxylase activity in vivo using the diagnostic substrate norcarane. Biotechnol. Article Google Scholar. Provided by the Springer Nature SharedIt content-sharing initiative, Over 10 million scientific documents at your fingertips, Not logged in Datsenko, K. A. D50, 760763 (1994). 2003 Fernandez-Martinez J, Pujalte MJ, Garcia-Martinez J, Mata M, Garay E, Rodriguez-Valeral F. Description of Alcanivorax venustensis sp. PHENIX: building new software for automated crystallographic structure determination. Gregson BH, Metodieva G, Metodiev MV, Golyshin PN, McKew BA. Attachment of Alcanivorax borkumensis to Hexadecane-In-Artificial Sea Water Emulsion Droplets. The protein and ligand samples were degassed before they were loaded into the cell and syringe. Filters were then incubated for 30min in scintillation fluid (ScintiVerse BD) and counted with a Packard Tri-Carb 1600TR liquid scintillation counter (Perkin Elmer, Waltham, MA). Biochim. CAS and E.W.Y. Enzymatic conversion of carbon atom 8 of guanosine triphosphate to formic acid. The current study aimed to use Alcanivorax borkumensis crude enzymes preparation as an agent for enhanced microbial hydrocarbons biodegradation in contaminated water and soil. The influence of calcium ions on properties of lipid bodies isolated from Alcanivorax borkumensis 10/2010 - 6/2011. USA 110, 20992104 (2013). 244, 15821592 (1969). Metallomics : integrated biometal science. FOIA . A team of European researchers has decoded the genome of a key oil-degrading bacterium, Alcanivorax borkumensis. We used ITC to examine the binding of sulfamethazine, sulfadiazine, sulfathiazole or sulfanilamide to the purified YdaH transporter. J. Bacteriol. Emsley, P. & Cowtan, K. Coot: model-building tools for molecular graphics. Pyne, C. & Bognar, A. L. Replacement of the folC gene, encoding folylpolyglutamate synthetase-dihydrofolate synthetase in Escherichia coli, with genes mutagenized in vitro. Long, F., Rouquette-Loughlin, C., Shafer, W. M. & Yu, E. W. Functional cloning and characterization of the multidrug efflux pumps NorM from Neisseria gonorrhoeae and YdhE from Escherichia coli. You searched for: Subject "Alcanivorax borkumensis" Remove constraint Subject: "Alcanivorax borkumensis" Start Over. Triglia, T., Menting, J. G., Wilson, C. & Cowman, A. F. Mutations in dihydropteroate synthase are responsible for sulfon and sulfonamide resistance in Plasmodium falciparum. 23, 979986 (1997). Ten l injections of the ligand solution were used for data collection. Chem. Int J Syst Bacteriol 48:339348. Filters were then incubated for 30min in scintillation fluid (ScintiVerse BD) and counted with a Packard Tri-Carb 1600TR liquid scintillation counter (Perkin Elmer, Waltham, MA). Learn more about Institutional subscriptions, Azapagic A, Emsley A, Hamerton I (2003) Polymers: the environment and sustainable development. Clin. borkumensis SK2. Marine hydrocarbonoclastic bacteria, like Alcanivorax borkumensis, play a globally important role in bioremediation of petroleum oil contamination in marine ecosystems.Accumulation of storage lipids, serving as endogenous carbon and energy sources during starvation periods, might be a potential adaptation mechanism for coping with nutrient limitation, which is a frequent stress factor . Bookshelf Front Microbiol. Alcanivorax borkumensis is a hydrocarbon degrading bacterium linked to oil degradation around oil spill sites. . Use several drops of the suspension to inoculate a #2698 agar slant and/or plate. These phases were then improved by NCS averaging with density modification using the programme RESOLVE38. Heterologous expression of the A. borkumensis AP1 alkB1 and alkB2 genes showed that they encode functional alkane hydroxylases with substrate ranges similar to those of their P. putida and P. aeruginosa homologues, indicating that the marine gamma-Proteobacterium Alcanivorax borks are regulated by a strategy different from that of the P.Putida . E. coli BL21(DE3)abgTpabA carrying pET15bydaH or pET15b were grown in LB broth with 100gml1 ampicillin at 37C. Kulathila, R., Kulathila, R., Indic, M. & van den Berg, B. The kanamycin-resistant gene was then released to generate the BL21(DE3)abgT knockout strain. Front Microbiol. 2008 Aug;285(1):89-96. doi: 10.1111/j.1574-6968.2008.01222.x. In this study, AlkB2 from A. borkumensis SK2 was expressed in Escherichia coli to examine the functionality of AlkB2 as a hydroxylating enzyme. CAS Google Scholar. The ion transporter superfamily. Calorimetry trials were also carried out in the absence of YdaH in the same experimental conditions. ', Paper presented at European School of Genetic Medicine, 1st Course in Microbial Bioremediation and Environmental Genomics, March 11-16, 2006, Bertinoro, Italy., 3/01/01. Filters were then incubated for 30min in scintillation fluid (ScintiVerse BD) and counted with a Packard Tri-Carb 1600TR liquid scintillation counter (Perkin Elmer, Waltham, MA). Acta 117, 275278 (1966). Semantic Scholar is a free, AI-powered research tool for scientific literature, based at the Allen Institute for AI. Measurements were performed on a VP-Microcalorimeter (MicroCal, Northampton, MA) at 25C. Production of a recombinant alkane hydroxylase (AlkB2) from Alcanivorax Escherichia coli abg genes enable uptake and cleavage of the folate catabolite p-aminobenzoyl-glutamate. Neutral lipid production in Alcanivorax borkumensis SK2 and other marine hydrocarbonoclastic bacteria. PubMed Bijan Bambai. Prasad M, Obana N, Sakai K, Nagakubo T, Miyazaki S, Toyofuku M, Fattaccioli J, Nomura N, Utada AS. It is likely that there is some limit to this model, but the limit was not determined in this paper. 2018. Hydrocarbon pads. The procedures for [3H]-sulfamethazine accumulation were the same as those for [3H]-PABA accumulation. Cells were then incubated for 10min, pelleted, washed twice and resuspended in the same buffer to OD600nm of 10. Antimicrob. Environmental microbiology 5; Applied microbiology and biotechnology 4; Langmuir 4; Proceedings of the National Academy of Sciences. Research Article. On the basis of . 1, 107125 (1999). When the OD600nm reached 0.5, the cultures were induced with 0.2mM IPTG and harvested for 2h after induction. Sci. Proc. Perspective Perspective: what is known, and not known, about the connections between alkane oxidation and metal uptake in alkanotrophs in the marine environment Sci. Meningococcal Disease John Wiley & Sons (1995). & Huennekens, F. M. Membrane-associated folate transport proteins. Triglia, T. & Cowman, A. F. Primary structure and expression of the dihydropteroate synthetase gene of Plasmodium falciparum. Environ Microbiol 6:264273, Article Reynolds, J. J. Proc. At various time points, samples of 100l were applied directly to prewetted glass-fibre filters, and washed twice with 5-ml aliquots of buffer containing 100mM Tris-HCl (pH 7.5). Disclaimer, National Library of Medicine PubMed Central 6:6874 doi: 10.1038/ncomms7874 (2015). Hyde, J. E. et al. Although, the sequence of alkB2 has been determined, the molecular characterization and the substrate specificity of AlkB2 require . Burg, A. W. & Brown, G. M. The biosynthesis of folic acid VI. CAS My first project sought to determine the effect of the alkane degrading microbe Alcanivorax . Cells were incubated with 75nM [3H]-sulfamethazine (Moravek Biochemicals, Brea, CA) for 15min, applied directly to prewetted glass-fibre filters and washed immediately twice with 5-ml aliquots of the same buffer. Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. D58, 17721779 (2002). Longitudinal tracking of biofilm-covered oil microdroplets using microfluidics reveals a spontaneous morphological transition from a thick biofilm phenotype to a thin dendritic phenotype optimized for high oil consumption rates. 180, 62606268 (1998). . 276, 307326 (1997). D Biol. 245, 30153025 (1970). Pathol. B. 2019 Jul 22;7(7):209. doi: 10.3390/microorganisms7070209. Genome sequence of the ubiquitous hydrocarbon-degrading marine bacterium Alcanivorax borkumensis. D54, 905921 (1998). Density-modified phases allowed us to visualize the helical structural features of the molecule. Nat Commun 6, 6874 (2015). Feasibility of treating emulsified oily and salty wastewaters through coagulation and bio-regenerated GAC filtration. B. Alcanivorax borkumensis is an oil-degrading marine bacterium. In this sense, the aim of this study was to establish a scale-up process using the constant oxygen mass transfer . Alcanivorax borkumensis is a cosmopolitan marine bacterium that uses oil hydrocarbons as its exclusive source of carbon and energy. Kaplan, J. D Biol. Acta Crystallogr. Institut fr Molekulare Mikrobiologie und Biotechnologie . All oligonucleotides were purchased from (Integrated DNA Technologies Inc., Coralville, IA) in a salt-free grade. Alcanivorax borkumensis, a marine bacterium highly specialized in degrading linear and branched alkanes, plays a key ecological role in the removal of marine oil spills.It contains several alternative enzyme systems for terminal hydroxylation of alkanes, including three P450 cytochromes (P450-1, P450-2 and P450-3). Transcriptional profiling of the marine oil-degrading bacterium Alcanivorax borkumensis during growth on n-alkanes. USA 94, 1394413949 (1997). It thrives in halophilic, aerobic environments and is found in the upper layers of freshwater or marine environments such as the Mediterranean Sea, Pacific Ocean and Arctic Sea [4, 8]. Chemother. J. Biol. Epub 2010 Mar 31. Richey, D. P. & Brown, G. M. The biosynthesis of folic acid. Chemical Papers 2017 Ostatn autoi. Crystallogr. The amphiphilic amphibactin siderophores produced by the oil-associated bacteria, Vibrio sp. Adams, P. D. et al. PLoS ONE 6, 15610 (2011). 187, 37133720 (2005). Researchers have genecally engineered other bacteria to consume petroleum . wrote the paper. Alcanivorax borkumensis (A. borkumensis) is a Gram-negative, rod-shaped hydrocarbonoclastic ("oil-degrading") bacterium. Alcanivorax borkumensis. Science 308, 13211323 (2005). Rev. Hara A, Baik SH, Syutsubo K, Misawa N, Smits TH, van Beilen JB, Harayama S. Environ Microbiol. TF6. Cloning and functional analysis of alkB genes in Alcanivorax borkumensis SK2. Biol. Emendations: Fernndez-Martnez et al. 31, 394395 (1999). The authors declare no competing financial interests. Commun. Crystallogr. Miri, M., Bambai, B., Tabandeh, F. et al. A. borkumensis is a biosurfactant producing bacteria as mentioned above. Proc Natl Acad Sci USA 104:56025607, Fujii T, Narikawa T, Takeda K, Kato J (2004) Biotransformation of various alkanes using the Escherichia coli expressing and alkane hydroxylase system from Gordonis sp. Neutral lipid production in Alcanivorax borkumensis SK2 and other marine hydrocarbonoclastic bacteria . Biosci Biotechnol Biochem 68:21712177, Golyshin PN, Martins dos Santos VAP, Kaiser O, Ferrer M, Sabirova YS, Lnsdorf H, Chernikova TN, Golyshina OV, Yakimov MM, Phler A, Timmis KN (2003) Genome sequence completed of Alcanivorax Purification and properties of the enzymes required for the formation of dihydropteroic acid. Due to the exponential nature of the model though, it is clear that the lag time decreases less rapidly after an initial concentration on the order of 10 8. The experiments were repeated for three times. Careers. Clin. borkumensis gen., nov., sp., nov., a new hydrocarbon-degrading and surfactant-producing marine bacterium. Google Scholar. J.R.B., C.-C.S., J.A.D., A.R., N.K., T.-H.C. and F.L. In this paper, we reported the crystal structure of the A. borkumensis YdaH transporter, revealing a dimeric molecule with a fold very distinct from other families of transporters. Alcanivorax borkumensis are prominent actors in oil spill bioremediation; however, the interfacial dynamics of their biofilms and its role in oil degradation remain unclear. Wiley, New York, pp 5254, Book government site. The overall focus of the research is to mutate mNeonGreen through random mutagenesis, a tool for modifying proteins, in order to improve its emission properties and/or discover novel properties of the fluorescent protein. J. Bacteriol. and transmitted securely. Crystallogr. Google Scholar. Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. CIP 105606. 239, 317325 (1964). Papers overview. Google Scholar. IX. need a perfect paper? Attachment of Alcanivorax borkumensis to Hexadecane-In-Artificial Sea Water Emulsion Droplets. Although, the sequence of alkB2 has been determined, the molecular characterization and the substrate specificity of AlkB2 require more precise investigation. Semantic Scholar uses AI to extract papers important to this topic. Alcanivorax borkumensis is whats called a halophilic organism, meaning it can survive in salt rich environments. Its genome contains genes coding for three cytochrome P450s and two integral membrane alkane hydroxylases (AlkB1 & AlkB2), all assumed to perform hydroxylation of different linear or branched alkanes. CAS Pronunciation of Alcanivorax borkumensis with 2 audio pronunciations and more for Alcanivorax borkumensis. 2004 Mar;6(3):191-7. doi: 10.1046/j.1462-2920.2003.00550.x. performed the experiments. Efran Manilla-Prez, Efran Manilla-Prez. We are very thankful to Marit Nilsen-Hamilton (Iowa State University) who generously made her radioactivity counter available for us. Genome sequence completed of Alcanivorax borkumensis, a hydrocarbon-degrading . place your first order and save 15% using coupon: Singer, M., Nambiar, S., Valappil, T., Higgins, K. & Gitterman, S. Historical and regulatory perspectives on the treatment effect of antibacterial drugs for community-acquired pneumonia. Wang, P., Read, M., Sims, P. F. G. & Hyde, J. E. Sulfadoxine resistance in the human malaria parasite Plasmodium falciparum is determined by mutations in dihydropteroate synthetase and an additional factor associated with folate utilization. HHS Vulnerability Disclosure, Help The site is secure. Microbial biodegradation is the use of bioremediation and biotransformation methods to harness the naturally occurring ability of microbial xenobiotic metabolism to degrade, transform or accumulate environmental pollutants, including hydrocarbons (e.g. Genome sequence of the ubiquitous hydrocarbon degrading marine bacterium Alcanivorax borkumensis. Woods, D. D. The relationship of p-aminobenzoic acid to the mechanism of the action of sulphanilamide. J. Biol. Ex-situ biodegradation of petroleum hydrocarbons using Alcanivorax borkumensis enzymes. Alcanivorax borkumensis are prominent actors in oil spill bioremediation; however, the interfacial dynamics of their biofilms and its role in oil degradation remain unclear. The mean enthalpies measured from injection of the ligand in the buffer were subtracted from raw titration data before data analysis with ORIGIN software (MicroCal). The multidrug efflux protein NorM is a prototype of a new family of transporters. 40, 658674 (2007). The concentrations of folic acid in BL21(DE3)abgTpabA expressing the mutant transporters were measured using the same procedures. Point Mutations Lead to Increased Levels of c-di-GMP and Phenotypic Changes to the Colony Biofilm Morphology in Alcanivorax borkumensis SK2. Crystallogr. Global topology analysis of the Escherichia coli inner membrane proteome. PubMedGoogle Scholar. By clicking accept or continuing to use the site, you agree to the terms outlined in our. 2022 Springer Nature Switzerland AG. Blue dextran (Sigma-Aldrich, St Louis, MO) was used to determine the column void volume. The https:// ensures that you are connecting to the Proc. 2015; Alcanivorax hongdengensis Wu et al. IV. Crystallography & NMR system: a new software suite for macromolecular structure determination. MeSH nov., a new, hydrocarbon-degrading and surfactant-producing marine bacterium. Species. Commun. In this paper, we provide background to the genome sequencing project of Alcanivorax borkumensis, which is a marine bacterium that uses exclusively petroleum oil hydrocarbons as sources of carbon and energy (therefore designated "hydrocarbonoclastic").
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