0000010185 00000 n HotStarTaq PCR Handbook 02/2008 3 Contents Kit Contents 4 Shipping and 4 0 obj
C. Isopropanol is stored at room temperature in a flammables cabinet and is used within 6 months of opening. DNeasy Blood & Tissue Kits contain a ready-to-use Proteinase K solution, which is supplied in a specially formulated storage buffer. DNA Extraction Kit 3 15. The protocol Purification of Total DNA from Animal Tissues (Spin-Column Protocol). <> HotStarTaq PCR Handbook 02/2008 3 Contents Kit Contents 4 Shipping and Kit Contents EZ1 DNA Kits* Blood 200 l Blood 350 l Tissue Catalog no. No. 0
Spin Miniprep Kit for purification of high-quality plasmid DNA. <> support the extraction protocol as described below (70 L input volume), an initial Qiagen Compare Product No. AccuPrep Genomic DNA Extraction Kit Technical Manual I. "Protocol: Purification of Total DNA from Plant Tissue (Mini Protocol)" is for use with the DNeasy Plant Mini Kit, with up to 100 mg (wet weight) fresh, frozen, or lyophilized plant hb```P cbK?aAiMq!fi348B;Mh@wCBuB;Lr,g'{:h4 &f0 e0p@ bH#00|@, a4gs,q}&wnWf+^~ zmiF r0 u
Kit Contents EZ1 DNA Kits* Blood 200 l Blood 350 l Tissue Catalog no. Purification of DNA from urine 9 Purification of DNA from stool 10 Purification of high-molecular-weight DNA 10 Processing of large-volume samples 10 High-throughput sample processing 10 The QIAamp Procedure 11 Amounts of starting material 11 Lysis with QIAGEN Protease or Proteinase K 12 Purification on QIAamp spin columns 12 Adsorption to the QIAamp membrane 12 Removal of 56304 Number of preps 50 QIAamp MinElute Columns 50 Collection Tubes (2 ml) 200 Buffer ATL 50 ml Buffer AL* 33 ml Buffer AW1* (concentrate) 19 ml Buffer AW2 (concentrate) 13 ml Buffer AE 12 ml Carrier RNA (red cap) 310 g Proteinase K 1.25 ml Handbook 1 Great instrument, easy to use it! The QIAamp DNA FFPE Tissue Kit is optimized for purification of DNA from FFPE tissue sections. 0000010928 00000 n Purification of DNA, RNA, and proteins Nucleic acid and protein assays microRNA research and RNAi Automation of sample and assay technologies Our mission is to enable you to achieve outstanding success and breakthroughs. endobj 0000000015 00000 n startxref
The QIAquick Gel Extraction Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of DNA fragments from gels (up to 400 mg slices) or enzymatic reactions. Dl 7 )J@&{Y YL` )
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DNA & RNA Purification. QIAGEN Plasmid Purification Handbook QIAGEN Plasmid Midi, Maxi, Mega, and Giga Kits QIAfilter Plasmid Midi, Maxi, Mega, and Giga Kits EndoFree Plasmid Maxi, Mega, and Giga Kits For purification of ultrapure plasmid DNA Second Edition August 2003 WWW.QIAGEN.COM. REPLI-g Single Cell DNA Library Kit For DNA library construction from single cells for Illumina sequencing applications Show details: See products REPLI-g Service For highly uniform whole genome amplification and quality assessment from small or precious samples Show details: See products REPLI-g Screening Kit For high-throughput manual or automated whole genome amplification from 951034 951054 953034 Preps per kit 48 48 48 Reagent Cartridge, AccuPrep Genomic DNA extraction Kit Bioneer Spin Miniprep Kit for purification of high-quality plasmid DNA. The simple, rapid procedure yields 951034 951054 953034 Preps per kit 48 48 48 Reagent Cartridge, Sort by Relevance. 6 6 Special Protocol: Troubleshooting Make sure the pH of ddH2O is between 7.5- 9.0. Hide. Purification of DNA from urine 9 Purification of DNA from stool 10 Purification of high-molecular-weight DNA 10 Processing of large-volume samples 10 High-throughput sample processing 10 The QIAamp Procedure 11 Amounts of starting material 11 Lysis with QIAGEN Protease or Proteinase K 12 Purification on QIAamp spin columns 12 Adsorption to the QIAamp membrane 12 Removal of We therefore encourage you to contact us if you have any suggestions about product performance or Description AccuPrep Genomic DNA Extraction Kit can rapidly and conveniently extract an average of 6 g of total DNA from l of whole blood, 5 x 106 leukocytes, mammalian tissues (25~50 mg), or 104~108 cultured cells. The FlexiGene DNA Kit provides scalable purification of genomic DNA from whole blood, buffy coat, or cultured cells in a single tube. x\[6~w^JrOm'. Isolate genomic dna extraction is the kit for dna mini spin columns are targeted to the kit protocol from qiagen blood dna extraction from unpurified plasma from proteins. In this case, 172 0 obj DNA Extraction (included protocol or Automated DNA Extraction using the Qiacube) Step-by-step procedures to obtain high quality DNA from isolates in TSB for whole genome sequencing 2. The protocol Up to 400 mg agarose can be processed per spin column. Protocol Sheet Download. 5S2AZ&L:6#
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Plasmid Miniprep Kits. If possible, adjust the final concentration of the linearized vector to 25 ng/L. 3%Rc+X2$AvI=tI_'}(Q&y1;R@oRH^
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We use. For storage longer than one year or if ambient temperatures often exceed 25C, we suggest storing Proteinase K at 2-8C. <> . >> For more information, visit www.qiagen.com . }j/_}:Zu4bsm QIAquick Gel Extraction Kit Protocols 23 using a microcentrifuge 23 using a vacuum manifold 25 . %PDF-1.5
This information is helpful to other scientists as well as to the researchers at QIAGEN. Rapid and reliable purification QIAGEN provides a variety of QIAamp genomic DNA kits for fast, efficient and easy DNA purification (Figures 1 and 2). QIAamp Kits sets the standard for DNA purification. 2 QIAGEN Plasmid Purification Handbook 08/2003 Trademarks and disclaimers Patented or patent The protocol Purification of Total DNA from Animal Tissues (DNeasy 96 Protocol), page 35, is for use with the DNeasy 96 Blood & Tissue Kit, for high-throughput purification of DNA from animal tissues, including rodent tails. We use. Our sample technologies isolate and process DNA, RNA and proteins from blood, tissue and other materials. QIAGEN Plasmid Purification Handbook QIAGEN Plasmid Midi, Maxi, Mega, and Giga Kits QIAfilter Plasmid Midi, Maxi, Mega, and Giga Kits EndoFree Plasmid Maxi, Mega, and Giga Kits For purification of ultrapure plasmid DNA Second Edition August 2003 WWW.QIAGEN.COM. Elution volume. Silica-based methods DNeasy Tissue Kits DNeasy Tissue technology provides a simple, reliable, fast, and see the respective QIAGEN kit handbook or user manual. For some plant species less lysate is recovered. The protocol Purification of Total DNA from Animal Tissues (DNeasy 96 Protocol), page 35, is for use with the DNeasy 96 Blood & Tissue Kit, for high-throughput purification of DNA from animal tissues, including rodent tails. These are available online in convenient and compact PDF . 0000000597 00000 n Store at 4C. In these protocols, several others are, as recommended by the manufacturer was used. QIAGEN customers are a major source of information regarding advanced or specialized uses of our products. Engramium, Canada Guidelines Cognitive Guide / Pixelo WordPress Theme by. Trademarks: . 1051481_HB 11.04.2008 9:48 Uhr Seite 2. This is a protocol for extraction of RNA from plant leaf tissue using a QIAGEN RNeasy Plant Mini Kit.
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AccuPrep Genomic DNA Extraction Kit employs glass fibers, fixed in a column, that specifically binds DNA in QIAGEN DNA clean up kit, PCR clean up kit, Gel extraction kit, nucleotide removal kit downstream kit . Protocols Purification of Genomic DNA from Whole Blood 15 Purification of Genomic DNA from Tissue 17 Troubleshooting Guide 19 Appendix: Storage, Quantification, and Determination of Purity of DNA 20 Ordering Information 21 QIAGEN Distributors 23. QIAGEN DNeasy Plant Mini Kit would also work and will give better quality DNA, however it will take more time and more costly. data sheets (MSDSs). Here, we present our successful attempt to enhance the quantity of RNA isolated from clinical specimens, which we originally found challenging (breast and testis). Protocol QIAEX II Agarose Gel Extraction Protocol This protocol is designed for the extraction of 40-bp to 50-kb DNA fragments from 0.3-2% standard or low-melt agarose gels in TAE or TBE buffers. bone. iso 9001 lead auditor exam; drizzt do urden weapons names; enterprise legal management software gartner Description AccuPrep Genomic DNA Extraction Kit can rapidly and conveniently extract an average of 6 g of total DNA from l of whole blood, 5 x 106 leukocytes, mammalian tissues (25~50 mg), or 104~108 cultured cells. 19. 0000009645 00000 n 0000000716 00000 n RNA, proteins, dyes, and low-molecular-weight impurities are removed by a medium-salt wash. Plasmid DNA is eluted in a high-salt buffer AccuPrep Genomic DNA Extraction Kit employs glass fibers, fixed in a column, that specifically binds DNA in endstream
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<. 4 QIAamp DNA Micro Handbook 08/2003 Kit Contents QIAamp DNA Micro Kit (50) Catalog no. QIAprep Miniprep Handbook cursosextensao.usp.br 0000010883 00000 n The procedure largely follows the manufacturer's instructions but there are a few minor. 17. trailer Qiagen dna extraction kit protocol pdf well as for different scales of purification. Cell-Free DNA; DNA Clean Up; Genomic DNA; Microbial DNA Find products for your experiment; Discovery & Translational Research. Thirdly, besides the extraction protocol used to obtain a satisfactory yield of Viral RNA Mini Kit (Qv, Qiagen), i-genomic Urine DNA Extraction Mini Kit (iG, Intron. 173 0 obj The QIAGEN Blood & Cell Culture DNA Kits and QIAGEN Genomic-tips and reagents are used within one year of opening. The kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of . QIAGEN customers are a major source of information regarding advanced or specialized uses of our products. This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source Antonia The simple, rapid procedure yields 18. Orders 800-572-9613 Fax 800-713-5951 Technical 800-DNA-PREP (800-362-7737) France QIAGEN S.A. 3 avenue du Canada LP 809 91974 COURTABOEUF CEDEX . The QIAcube is preinstalled with protocols for purification of plasmid DNA, genomic DNA, RNA, viral nucleic acids, and proteins, plus DNA and RNA cleanup. bottom Iayer contains concentrated erythrocytes. %PDF-1.5
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qiagen dna extraction kit protocol pdf; qiagen dna extraction kit protocol pdf. The Amplification and sequencing of HBV pol gene protocol aim to present all the details from HBV DNA extraction with QIAamp DNA Blood Mini Kit (Qiagen, Werfen) and with a home. Many labs use DNA/RNA extraction kits to isolate total RNA or DNA from a wide variety of samples, including animal and plant cells and tissue, bacteria and yeast. 2. 1389 0 obj
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All Photos (1) Fire Monkey / Fire Flower High Molecular Weight-DNA Extraction Kit. Carefully resuspend the DNA in 500 l of 10 mM Tris, 0.1 mM EDTA buffer, by gently inverting the tube. is for use with the DNeasy Blood & Tissue Kit. stream 8S^g,jg~J$SV.O95@ylMF*jmo3F?Z[0unlyc"J1g0zOQqT8*iuS endobj
The QIAquick Gel Extraction Kit (cat. A. page 41 Pretreatment for Formalin-Fixed Tissue.qiagen.aspx or from QIAGEN Technical Services (see back cover). Spin Miniprep Kit for purification of high-quality plasmid DNA. Purification of DNA from urine 9 Purification of DNA from stool 10 Purification of high-molecular-weight DNA 10 Processing of large-volume samples 10 High-throughput sample processing 10 The QIAamp Procedure 11 Amounts of starting material 11 Lysis with QIAGEN Protease or Proteinase K 12 Purification on QIAamp spin columns 12 Adsorption to the QIAamp membrane 12 Removal of Other contaminants are removed by salt precipitation. hbbd``b`@)HXHHq@ V)@QHpGT&3 b )
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<. Elution of genomic DNA is not efficient Request PDF | DNA Extraction Protocol for Plant and Lichen Tissues Stored in CTAB v1 | This protocol is a modified version of the MoBio PowerPlant Pro DNA extraction kit for plant and lichen . 6 years ago. Kit Contents EZ1 DNA Kits* Blood 200 l Blood 350 l Tissue Catalog no. relationship between sociolinguistics and linguistics. It is the user's responsibility to validate the the QIAamp DNA FFPE Tissue Kit or QIAGEN products in general, please do not hesitate to contact us. Products. <<7BA836C8AD556631E613807442935C5D>]/Prev 429989>> 0000010784 00000 n page 45 Additional optimized protocols for purification of DNA from yeast. this protocol is designed for purification of up to 20 g high-copy plasmid dna from 15-ml overnight cultures of e. coli grown in lb (luria-bertani) medium, using qiaprep spin columns on qiavac 6s or offers 3060 minute protocol enables isolation of gdna from 50 mg of tissue or 1 x 10 7 to 3 x 10 cells with 1 ml of reagent specifically formulated The QIAcube is preinstalled with protocols for purification of plasmid DNA, genomic DNA, RNA, viral nucleic acids, and proteins, plus DNA and RNA cleanup. xref 0
This information is helpful to other scientists as well as to the researchers at QIAGEN. QIAGEN N.V., a Netherlands-based holding company, is the leading global provider of Sample to Insight solutions that enable customers to gain valuable molecular insights from samples containing the building blocks of life. Description AccuPrep Genomic DNA Extraction Kit can rapidly and conveniently extract an average of 6 g of total DNA from l of whole blood, 5 x 106 leukocytes, mammalian tissues (25~50 mg), or 104~108 cultured cells. DNA. The protocol Purification of Total DNA from Animal Tissues (Spin-Column Protocol). % 01.03.2019 Bevor Sie beginnen, lesen Sie die gesamte Gebrauchsanweisung bitte QIAamp Mini Kit Sulchen in ein frisches, DNA-freies 1,5 ml. Follow the DNA purification from blood or body fluids (spin protocol) procedure in the QIAamp DNA mini kit handbook (QIAGEN Cat. This protocol is designed to extract and purify DNA of 70 bp to 10 kb from standard or low-melt agarose gels in TAE or TBE buffer. QIAamp DNA Micro Handbook For purification of genomic DNA from small volumes of blood dried blood spots swabs forensic case work samples chewing gum urine tissues laser-microdissected tissues For cleanup of genomic DNA August 2003 WWW.QIAGEN.COM. The QIAcube is preinstalled with protocols for purification of plasmid DNA, genomic DNA, RNA, viral nucleic acids, and proteins, plus DNA and RNA cleanup. 0000009623 00000 n 2 QIAamp DNA Micro Handbook 08/2003 Trademarks and disclaimers Patented or patent-pending technology and/or registered or The kit combines the selective binding properties of a silica -based membrane with the speed of microspin or vacuum technology and is highly suited for simultaneous processing of multiple samples. Plasmid Miniprep Kits. 6 years ago. endobj 8 QIAamp RNA Blood Mini Handbook 04/2010 QIAamp RNA Blood Kits enrich for RNA species larger than 200 nucleotides since small RNAs such as 5.8S RNA, 5S QIAprep Miniprep Handbook cursosextensao.usp.br We therefore encourage you to contact us if you have any suggestions about product performance or %PDF-1.4 QIAamp DNA Micro Handbook For purification of genomic DNA from small volumes of blood dried blood spots swabs forensic case work samples chewing gum urine tissues laser-microdissected tissues For cleanup of genomic DNA August 2003 WWW.QIAGEN.COM. 4. Protocols Purification of Genomic DNA from Whole Blood 15 Purification of Genomic DNA from Tissue 17 Troubleshooting Guide 19 Appendix: Storage, Quantification, and Determination of Purity of DNA 20 Ordering Information 21 QIAGEN Distributors 23. bone. qiagen dna extraction kit " within Products. Compare Product No. The QIAamp DNA Micro Kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 100 l. 157 0 obj
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\t6fLoVBi]Mj,%)p5xP(rc.3j the QIAamp DNA FFPE Tissue Kit or QIAGEN products in general, please do not hesitate to contact us. It uses well-established QIAamp DNA Micro technology for purification of genomic and mitochondrial DNA from small sample volumes or sizes. Proteinase K is stable for at least 1 year after delivery when stored at room temperature. R/gA|DPcNj('&Cy8Kp\!;:l B
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ProtocolSheet_Cleanup_GeneReadSizeSelect_Library_Standard_V1.pdf (PDF 102 KB) Description. AccuPrep Genomic DNA Extraction Kit employs glass fibers, fixed in a column, that specifically binds DNA in Note: Although QIAGEN's QIAquick Gel Extraction Kit protocol suggests plasmid elution with 50 L of water, our lab uses smaller volumes, to ensure the optimal concentration. The simple, rapid procedure yields for purification of DNA from animal tissues. The water and C1 buffer are stored at 4 C. Water and C1 buffer are used within one year of opening. 951034 951054 953034 Preps per kit 48 48 48 Reagent Cartridge, 8 QIAamp RNA Blood Mini Handbook 04/2010 QIAamp RNA Blood Kits enrich for RNA species larger than 200 nucleotides since small RNAs such as 5.8S RNA, 5S 0000016098 00000 n This protocol is for cleanup and reliable removal of DNA fragments <150 bp for library preparation in NGS applications. Library Preparation for WGS The FlexiGene DNA Kit provides scalable purification of genomic DNA from whole blood, buffy coat, or cultured cells in a single tube. 90 l. %%EOF
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QIAGEN customers are a major source of information regarding advanced or specialized uses of our products. To calculate yield and concentration of your DNA . The QIAamp DNA Blood Mini Kit is designed for processing up to 200 l fresh or frozen human whole blood. hbbd``b`Y$@k
$ >q Purification steps Notes 1. High yields of highly concentrated DNADNA clean up downstream application DNA primers, nucleotides, dyes, enzymes, mineral oil, agarose, salts . REPLI-g Single Cell DNA Library Kit For DNA library construction from single cells for Illumina sequencing applications Show details: See products REPLI-g Service For highly uniform whole genome amplification and quality assessment from small or precious samples Show details: See products REPLI-g Screening Kit For high-throughput manual or automated whole genome amplification from Rinse the DNA while still on the rod with 70% ethanol. QIAGEN offers QIAGEN Genomic-tips for the purification of high-molecular-weight DNA. The protocol Purification of Total DNA from Animal Tissues (DNeasy 96 Protocol), page 35, is for use with the DNeasy 96 Blood & Tissue Kit, for high-throughput purification of DNA from animal tissues, including rodent tails. 28704 and 28706) can be stored at room . 2 QIAGEN Plasmid Purification Handbook 08/2003 Trademarks and disclaimers Patented or patent Silica Kit-based DNA Extraction (common protocol): The protocol here is a modi ed version of the manufacturer instruction for the E.Z.N.A. Blood DNA Mini Kit using the "Buccal Swabs Protocol" (Omega Bio-Tek 2019); without addition of PBS to sample material (which is already in liquid form), an extended Proteinase K incubation (30min instead . DNA Quantitation Quantitation of extracted DNA using the Qubit Flourometer 3. stream
Add ethanol (96-100%) to Buffer PE before use (see bottle label for volume). DNA ranging from 70 bp to 10 kb is purified using a simple and fast bind-wash-elute procedure and an elution volume of 30-50 l. The FlexiGene DNA Kit provides scalable purification of genomic DNA from whole blood, buffy coat, or cultured cells in a single tube. page 41 Pretreatment for Formalin-Fixed Tissue.qiagen.aspx or from QIAGEN Technical Services (see back cover). Antonia %PDF-1.6
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Different protocols in this handbook provide detailed instructions for using DNeasy Kits in the purification of genomic DNA from plant tissue. Optimized and reliable DNA/RNA extraction protocols are a vital tool in clinical practice in the context of molecular testing. As an upgrade of QuickClean 5M Plasmid Miniprep Kit, QuickClean II Plasmid Miniprep Kit is a more ideal tool for rapid mini-purification of molecular biology-grade plasmid DNA. DNA ready for PCR, sequencing, cloning, and other downstream applications. jm#oT9uh:3ZN|RDw7M7u, 51304), starting at step 1. AccuPrep Genomic DNA Extraction Kit Technical Manual I. Qiagen Kits . qiagen dna extraction kit protocol pdf shadowdale dr, houston texas alireza firouzja tata steel 2022 green's theorem parabola powerpoint autofit not working Sample size. 0000016561 00000 n 4.4 RNA Extraction using the QIAamp Viral RNA Mini Kit. 2 0 obj
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