Ying Yong Sheng Tai Xue Bao. Applied Environmental Microbiology. Ying Yong Sheng Tai Xue Bao. ISME J, Romero IC, Toro-Farmer G, Diercks A-R, Schwing P, Muller-Karger F, Murawski S, Hollander DJ (2017) Large-scale deposition of weathered oil in the Gulf of Mexico following a deep-water oil spill. Bagby SC, Reddy CM, Aeppli C, Fisher GB, Valentine DL (2017) Persistence and biodegradation of oil at the ocean floor following Deepwater Horizon. 2010 Nov 15;183(1-3):395-401. doi: 10.1016/j.jhazmat.2010.07.038. 1997 Jan;43(1):17-22 The emulsification activity was calculated as follows: Different techniques were used to isolate and estimate the amount of biosurfactants produced by D. spicifera CFSs. Their large-scale production can be achieved under severe environmental conditions, such as stress [9]. An official website of the United States government. In the current study, and for the first time, we evaluated the bio-degradation capabilities of fungus D. spicifera, isolated from contaminated soil samples in crude oil reservoirs in Riyadh, Saudi Arabia. Comparison with the untreated control showed an increase of the DCPIP color absorbance at 450 nm in all tested hydrocarbons (Table 2). Biodegradation of petroleum hydrocarbons by oleophilic strain of Pseudomonas aeruginosa NCIM 5514. The culture was also effective as an inoculant for the bioaugmentation of total petroleum hydrocarbon-contaminated soil and as a de-emulsifier of oilfield emulsions and could transform some other environmental contaminants which are not predominant components of crude oil. Briefly, 100 seeds of tomato were pre-treated with sodium hypochlorite, washed three times with distilled water, then soaked overnight in 50 mL of CFS with shaking. Finally, to test the toxic effects of biosurfactants produced by D. spicifera, their germination activity was explored using tomato seeds for twenty days. Furthermore, the filamentous structure of fungi allows their easy separation through filtration [8]. https://doi.org/10.1016/j.marpolbul.2016.04.044, Rodriguez-r LM, Overholt WA, Hagan C, Huettel M, Kostka JE, Konstantinidis KT (2015) Microbial community successional patterns in beach sands impacted by the Deepwater Horizon oil spill. Furthermore, the SEM images of D. spicifera showed morphological changes in the structure of the isolates treated with 1% crude oil (Figure 3). Advances in research on petroleum biodegradability in soil. Ecotoxicol Environ Saf. Biodegradation alone can include a lot of the D. spicifera or Bipolaris spicifera is the taxonomical nomenclature of a fast-growing seed-borne fungus. 1999 Jan;51(1):105-11 Rev Sci Instrum 85(5):053101. https://doi.org/10.1063/1.4873195, Nunoura T, Soffientino B, Blazejak A, Kakuta J, Oida H, Schippers A, Takai K (2009) Subseafloor microbial communities associated with rapid turbidite deposition in the Gulf of Mexico continental slope (IODP Expedition 308). PubMedGoogle Scholar. Biodegradation of Petroleum Hydrocarbons. Our results show a significant increase in the seeds germination, which reflects their higher environmental safety levels. Science 330(6001):204208. https://doi.org/10.1146/annurev-marine-010814-015543, Kleindienst S, Seidel M, Ziervogel K, Grim S, Loftis K, Harrison S, Malkin SY, Perkins MJ, Field J, Sogin ML, Dittmar T, Passow U, Medeiros PM, Joye SB (2015a) Chemical dispersants can suppress the activity of natural oil-degrading microorganisms. PLoS One 10:124, Camilli R, Reddy CM, Yoerger DR, Van Mooy BAS, Jakuba MV, Kinsey JC, McIntyre CP, Sylva SP, Maloney JV (2010) Tracking hydrocarbon plume transport and biodegradation at Deepwater Horizon. A light-brown-colored precipitate was formed and collected by spinning down at 10,000 rpm, 4 C for 30 min. ), followed by used oil (0.514 a.u. The degradation of petroleum hydrocarbons can be mediated by specific enzyme systems. The ability of D. spicifera to tolerate effects of different hydrocarbons in liquid medium. Removal of petroleum hydrocarbon pollutants from environment by applying oleophilic microorganisms (individual isolate/consortium of microorganisms) is ecofriendly and economic. eCollection 2015. The acid precipitation assay showed that the amount of the produced precipitate differed according to the type of hydrocarbon (Figure 5A). 2005 Jan;58(4):515-22. doi: 10.1016/j.chemosphere.2004.09.021. Thus, the DWH incident raised new challenges with regard to predictions of petroleum hydrocarbon (PHC) biodegradation and the fate of discharged hydrocarbons in the deep sea, which is permanently cold (~4C) and exposed to high hydrostatic pressure (1 MPa per 100m). Fresh oil hydrocarbons were obtained from Aramco Co. (Dammam, Saudi Arabia) in sterile bottles. at 450 nm, of, Drop-collapse test to liquid contains surfactants from different strain of, Varjani S.J., Upasani V.N. Science 349(6248):592593. Previous studies have showed that the fungi of Drechslera sp., Fusarium sp., and Papulaspora sp. Disclaimer, National Library of Medicine Petroleum hydrocarbon pollutants are recalcitrant compounds, ranked as priority pollutants [2]. A piece of fungal hyphae of D. spicifera (1 cm2) was added to each hydrocarbon-specific mixture and incubated on a shaker at 25 C for two weeks. A previous study has been conducted on petroleum hydrocarbons contaminated soil samples from India, and identified the presence of Drechslera halodes, which causes hydrocarbon enzymatic bioremediation by 37 0.7 U/mL of extracellular lipase [24]. NCI CPTC Antibody Characterization Program, Appl Environ Microbiol. In a study from India, Pseudomonas aeruginosa showed an efficient production of biosurfactant with crude oil, which degraded ~82% of the petroleum hydrocarbons after 35 days [12]. In particular, hydrostatic pressure is a key environmental parameter that has been largely overlooked in biodegradation studies, due to methodological challenges and the difficulty to obtain samples. Science 330(6001):208211. oil), polychlorinated biphenyls (pcbs), polyaromatic hydrocarbons (pahs), heterocyclic RSP-2021/114/the Researchers Supporting Project, King Saud University, Riyadh, Saudi Arabia, Varjani S.J., Upasani V.N. The ePub format uses eBook readers, which have several "ease of reading" features 1999; Van Hamme et al. The site is secure. The .gov means its official. Proc Natl Acad Sci 114(1):E9, CrossRef Deep-Sea Res II 57:20082021, Overholt W (2018) The response of marine benthic microbial populations to the Deepwater Horizon oil spill, Ph.D. Dissertation, Georgia Institute of Technology, pp 248, Passow U, Ziervogel K, Asper V, Diercks A (2012) Marine snow formation in the aftermath of the Deepwater Horizon oil spill in the Gulf of Mexico. The bio-catalytic action of HYDROKING immediately biodegrades the molecules of hydrocarbon oil, grease, diesel, petrol, ballast bunker oil and other organics hydrocarbons. Mycobiota Associated with Imported Seeds of Vegetable Crops in Sudan. The https:// ensures that you are connecting to the Biodegradation of a mixture of PAHs by non-ligninolytic fungal strains isolated from crude oil-contaminated soil. Sajna K.V., Sukumaran R.K., Gottumukkala L.D., Pandey A. The production of biosurfactant by . It achieved this by using different assays. MeSH [Bioremediation of petroleum hydrocarbon-contaminated soils by cold-adapted microorganisms: research advance]. Parthipan P., Preetham E., Machuca L.L., Rahman P.K., Murugan K., Rajasekar A. Biosurfactant and Degradative Enzymes Mediated Crude Oil Degradation by Bacterium, Khandelwal A., Singh S.B., Sharma A., Nain L., Varghese E., Singh N. Effect of surfactant on degradation of. In the current study, the ability of biosurfactants produced by D. spicifera to increase the oil emulsification was tested [55]. Briefly, MSM culture media was mixed with 1% of one of the tested hydrocarbons in addition to 0.1% Tween 80 and DCPIP (0.6 mg/mL). Another study from Saudi Arabia used a mixture of biosurfactants produced by Alternaria alternata, Aspergillus flavus, Aspergillus terreus, and Trichoderma harzianum, which caused successful biodegradation (73.6%) of crude oil [40]. The fungal growth effects on the physical characteristics, Bio-surfactant recovery assays of D. spicifera . Six soil samples were collected from polluted crude oil reservoirs in two main districts, Al Faisaliyyah (two samples) and Ghubairah (four samples), in Riyadh city, Saudi Arabia. 2016). Hentati D, Abed RMM, Abotalib N, El Nayal AM, Ashraf I, Ismail W. Front Microbiol. The site is secure. Several studies have shown the ability of some fungal species to produce degrading enzymes, such as catalases, peroxidases, and laccases, which increase the degradation and immobilization of organic and inorganic contaminants [35,36]. Curr Opin Microbiol 1(3):291295, Jrgensen BB, Boetius A (2007) Feast and famine microbial life in the deep-sea bed. PMC 2020 May;129(5):603-612. doi: 10.1016/j.jbiosc.2019.12.001. This article provides an overview about bioremediation for petroleum hydrocarbon pollutants. For example, the buffering oil), polychlorinated biphenyls (pcbs), polyaromatic hydrocarbons (pahs), heterocyclic The toxicity levels of isolated biosurfactants were assessed by testing their effect on the germination of Solanum lycopersicum (tomato) seeds against water (as a control) [59,60]. The hydrocarbon tolerance capacity was estimated by calculating the mycelial growth and DIR for ten days post-inoculation. Somee MR, Amoozegar MA, Dastgheib SMM, Shavandi M, Maman LG, Bertilsson S, Mehrshad M. BMC Genomics. Germination assay of D. spicifera. The ability of the fungal strain to use different hydrocarbons as a sole source of carbon was tested by testing the growth rate on solid and liquid medium. These samples included crude oil, kerosene, diesel, and used oil. Similarly, the solvent extraction assay showed that crude oil was the highest (5.25 g), followed by used oil (4.66 g), where the lowest production was for mixed oil (1.53 g). Enhanced bioremediation of oil spills in the sea. 2022 Aug 9. doi: 10.1007/s00449-022-02763-3. It also includes explanation about hydrocarbon metabolism in microorganisms with a special focus on new insights obtained during past couple of years. The authors declare no conflict of interest. Appl Environ Microbiol 70(2):12311233. Paza G.A., Zjawiony I., Banat I.M. Another study has shown that D. spicifera isolated from three oil stations in Egypt could degrade solid anthracene (28.6%) and the water-soluble fractions of crude oil (66.21%) after 14 days [21]. -, Appl Microbiol Biotechnol. 2016;222:195201. Another study showed a correlated higher surface activity of biosurfactant solution with the larger diameter produced by Lactococcus lactis [32]. A study conducted by Ting et al., (2009) reported that Pseudomonas lundensis produced clearing zones when incubated with paraffin, mineral, and crude oils by metabolizing the long-chain-length of alkanes [27]. 2016 Dec;222:195-201. doi: 10.1016/j.biortech.2016.10.006. 8600 Rockville Pike This site needs JavaScript to work properly. In different ecosystems, specific microorganisms have been reported to degrade different groups of molecules. Limnol Oceanogr Methods 9(10):499506. Bookshelf Offshore Technology Conference, 6-9 May, Houston, Texas, USA 2013 May 6, 2013. When compared with the control, all hydrocarbons resulted in higher growth of D. spicifera. Contrasting physiological mechanisms were observed for different component organisms of the mixed culture with respect to their associations with the hydrocarbon substrate; and the beneficial effects of using surfactants were demonstrated. Degradation of crude oil by mixed cultures of bacteria isolated from the Qinghai-Tibet plateau and comparative analysis of metabolic mechanisms. These findings highlight the biodegradation ability of D. spicifera, which has been proven in the use of petroleum oils as the sole source of carbon. In order to resolve the issue of increased costs and limited efficiency of conventional physicochemical methods, alternative technologies for in situ applications have developed, specifically, techniques based on the biological remediation capacity of plants and microorganisms [1,3]. Guglielminetti M., De Giuli Morghen C., Radaelli A., Bistoni F., Carruba G., Spera G., Caretta G. Mycological and Ultrastructural studies to evaluate biodeterioration of mural paintings. 2014 Jun;98(11):4781-94. doi: 10.1007/s00253-014-5684-9. Licensee MDPI, Basel, Switzerland. Other mechanisms involved are (1) attachment of microbial cells to the substrates and (2) production of biosurfactants. Springer, Cham. F.A.-O. was induced by crude oil on the 15th day. The most significant characteristic of a potential hydrocarbon degrader is the ability to produce microbial biosurfactants, which compromise a mixture of organic (proteins, fatty acids, exopolysaccharides, and amino acids) and inorganic components [33]. Epub 2014 Mar 19. Biodegradation of petroleum hydrocarbons is a complex process that depends on the nature and on the amount of the hydrocarbons present. Krawczyska M., Kowzan B., Rybak J., Gediga K., Shcheglova N.S. Curr Opin Biotechnol. Keywords: 2015;103:116124. and N.M. contributed to the conception, study design, and the editing and reviewing of the intellectual contents. Bioremediation potential of hydrocarbon-utilizing fungi from select marine niches of India. Latha R., Kalaivani R. Bacterial degradation of crude oil by gravimetric analysis. Adebajo S., Akintokun P., Ojo A., Akintokun A., Badmos O. Bioremediation potential of hydrocarbon-utilizing fungi from select marine niches of India. https://doi.org/10.1126/science.1199697, Kimes NE, Callaghan AV, Suflita JM, Morris PJ (2014) Microbial transformation of the Deepwater Horizon oil spill past, present, and future perspectives. The term is often referred to as the process of biodegradation. They naturally biodegrade pollutants and thereby remove them from the environment. The tubes were homogenized at high speed and incubated overnight at room temperature. Cell 172(6):13361336.e1331. Yang R, Zhang G, Li S, Moazeni F, Li Y, Wu Y, Zhang W, Chen T, Liu G, Zhang B, Wu X. Environ Sci Pollut Res Int. Biodegradation > 2019 > 30 > 4 > 351-361. http://www.nature.com/ngeo/journal/v4/n3/abs/ngeo1067.html#supplementary-information, Joye SB, Teske AP, Kostka JE (2014) Microbial dynamics following the Macondo oil well blowout across Gulf of Mexico environments. Llad S., Covino S., Solanas A.M., Vias M., Petruccioli M., Dannibale A. Similarly, the soil samples collected from contaminated oil stations in the Suez Gulf in Egypt, indicated the existence of D. spicifera in the water bottom and sediment of the samples [21]. Epub 2020 Jan 25. However, the biodegradation of crude oil-contaminated sludge could be a time-consuming and low-efficiency process.